UJI EFEKTIVITAS EKSTRAK BUAH ANGGUR HIJAU (VITIS VINIVERA L) TERHADAP DAYA HAMBAT LAJU PERTUMBUHAN BAKTERI STREPTOCOCCUS MUTANS DAN LACTOBACILLUS ACIDOPHILUS

Streptococcus mutans and lactobacillus are acid-producers of carbohydrates
that can be fermented and create the risk for cavity. Green grapes (Vitis vinifera L) is
one of the fruit variety that contains the phytochemical from polyphenols class which
functions as an inhibitors of glucosylation on plaque formation so that it was used as
an antibacterial alternative in caries prevention. The purpose of this research was to
determine the effectiveness of green grapes antibacterial towards Streptococcus
mutans and lactobacillus acidophilus. The research design that used in this study was
a laboratory experimental (Invitro).This research was conducted in Microbiology
Laboratory of Andalas University, Padang City and the maceration was conducted in
chemistry laboratoryKopertis region X (West Sumatra, Riau, Jambi, Riau Islands)
Padang City, then the maceration is done in the Microbiology Laboratory of Andalas
University Padang City on July 2019 until finished. The Samples on this research were
the colonies of Streptococcus mutans bacteria and Lactobacillus acidophilus which
obtained from the Laboratory of Andalas University, Padang city and Gajah Mada
University and then they were bred at the Microbiology Laboratory of Andalas
University Padang city with 4 treatments done and repeated as well 6 times so that the
number of the samples were 24 treatments with the precentage of concentration
12.5%, 25%, 50%, and 100% by used one way anova test. The results of the research
showed the obtained number of sig 0.000 <0.05, it means that Ha received or it found
the effectiveness of green grapes extracts (Vitis vinifera L) towards the inhibition
capacity of Streptococcus mutants bacterium rate growth with best extract 50%, and
the number of sig 0.244> 0.05, it means Ha was rejected or did not found the
effectiveness of green grapes extracts(Vitis vinifera L) towards the inhibition of
Lactobacillus Acidophilus rate growth.