ISOLATION AND IDENTIFICATION OF PATHOGENIC BACTERIA SECRETION OF CHRONIC SUPPURATIVE OTITIS MEDIA PATIENTS

The aims of this research were to isolate and identify the pathogenic bacteria in the secretion of Chronic Suppurative Otitis Media (CSOM) patients as the development of Lactic Acid Bacteria (LAB) analysis in Virgin Coconut Oil (VCO) fermentation process. It is expected that LAB in the VCO could be antimicrobial/antibacterial of bacteria in the secretion of CSOM patients. There were 2 stages conducted within this research, firstly using blood agar and dilution method in isolating the bacteria in CSOM patients’s secretion. Secondly, the isolates were identified morphologically, physiology, and another biochemical test. There are 126 isolates and 5 kinds of pathogenic bacteria(Pseudomonas aureginosa, Staphilococus aureus, Staphilococusepidermidis, Proteus mirabilis,Klebsiella Sp) and one kind of fungi (Candida sp)as the result. The samples of CSOM patients are 60% above aged 20 and 40% below it, and an equal balance of percentage between male and female. Keywords: Pathogenic bacteria isolation, Secretion of CSOM patients, Chronic Suppurative OtitisMedia, Virgin Coconut Oil (VCO), Lactic Acid Bacteria (LAB).