Phyto Effect of Punica granatum on the Remodelling of Maxilla Bone, Study of Osteoblast and Osteoclast

Abstract. Punica granatum (PG) contained anthocyanin, the chemical compound that played
significant role in increasing bone cell proliferabbitsion and osteoblasts differentiation in bone
remodelling. This research was aimed at measuring the effect of Punica granatum on maxilla bone
remodelling relating to the profile of both osteoblast and osteoclast. Punica granatum was extracted
by using butanol while the analysis of bone remodelling was conducted by observing the profile of
osteoblast and osteoclast cells using histopathologic method. Butanol hydrogel of Punica granatum
was abled to enhance bone reabsorption which was proven by the increase of osteoclast and new bone
formation since the osteoblast was presented. Moreover, bone remodelling activity was characterised
by increased collagen fibres and osteocyte cell, in addition, bone shape compactness was getting
better. Statistical analysis revealed that the histoscore profile of osteoblast was influenced by butanol
fraction dosage with significance level (p<0.05) which was at medium criteria according to Pearson
correlation (r=0,6). While the effect of dosage on osteoclast was at significance level (p<0.05) but
was poorly correlated with (r=0.01) and osteoblast frequency was dominant at the concentrabbitsion
of 125 mg/ml and 250 mg/ml. On the other hand, at the concentrabbitsion of 175 mg/ml osteoclast
presentation was found to be more dominant. The fraction of butanol PG was abled to induce
osteoblast increment and osteoclast at the concentrabbitsion of (mg/ml) 125, 175, and 250 and
accelerabbitse the remodelling of alveolar maxilla.